polyclonal rabbit anti mouse langerin antibody (Novus Biologicals)
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Polyclonal Rabbit Anti Mouse Langerin Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyclonal+rabbit+anti+mouse+langerin+antibody/Rabbit+anti-Mouse+IgG2b+Secondary+Antibody/pmc04471117-223-23-29
Average 92 stars, based on 7 article reviews
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1) Product Images from "Papain Degrades Tight Junction Proteins of Human Keratinocytes In Vitro and Sensitizes C57BL/6 Mice via the Skin Independent of its Enzymatic Activity or TLR4 Activation"
Article Title: Papain Degrades Tight Junction Proteins of Human Keratinocytes In Vitro and Sensitizes C57BL/6 Mice via the Skin Independent of its Enzymatic Activity or TLR4 Activation
Journal: The Journal of investigative dermatology
doi: 10.1038/jid.2015.58
Figure Legend Snippet: Skin sections were embedded in paraffin and stained (n = 4–5 per group) with anti-Langerin (a), and anti-Ly-6G (b) and anti-CCL8 antibody (c), scanned, and evaluated using Histoquest analysis program. The epidermis and dermis were evaluated separately. Median+Max./Min.; *P<0.05; **P<0.01; ***P<0.001. Max., maximum; min., minimum; WT, wild type.
Techniques Used: Staining
Related Articles
Staining:Article Title: Papain Degrades Tight Junction Proteins of Human Keratinocytes In Vitro and Sensitizes C57BL/6 Mice via the Skin Independent of its Enzymatic Activity or TLR4 Activation Article Snippet: Immunohistochemistry Tissues were deparaffinized and treated using 30 U ml −1 Proteinase K in PBS (Sigma) for 5 minutes at 37 °C, followed by standard protocols (Santa Cruz Biotechnology, ImmunoCruz ABC staining system kits; online repository).Immunohistochemistry Tissues were deparaffinized and treated using 30 U ml −1 Proteinase K in PBS (Sigma) for 5 minutes at 37 °C, followed by standard protocols (Santa Cruz Biotechnology, ImmunoCruz ABC staining system kits; online repository).. Primary antibodies were as follows: rat anti-mouse Ly-6G antibody 1:100 (Novus Biologicals, Littleton, CO, RB6-8C5), polyclonal rabbit anti-mouse CCL8/MCP-2 antibody 1:100 (antibody-online), and polyclonal rabbit anti-mouse Langerin antibody 1:250 (Novus Biological), controls: rat IgG2b, rabbit IgG.. Primary antibodies were incubated over night at 4 °C (Ly-6G and CCL8) or for 2 hours at room temperature (Langerin).Primary antibodies were incubated over night at 4 °C (Ly-6G and CCL8) or for 2 hours at room temperature (Langerin). Article Title: Papain Degrades Tight Junction Proteins of Human Keratinocytes In Vitro and Sensitizes C57BL/6 Mice via the Skin Independent of its Enzymatic Activity or TLR4 Activation Article Snippet: Tissues were deparaffinized and treated using 30 U ml −1 Proteinase K in PBS (Sigma) for 5 minutes at 37 °C, followed by standard protocols (Santa Cruz Biotechnology, ImmunoCruz ABC staining system kits; online repository).Tissues were deparaffinized and treated using 30 U ml −1 Proteinase K in PBS (Sigma) for 5 minutes at 37 °C, followed by standard protocols (Santa Cruz Biotechnology, ImmunoCruz ABC staining system kits; online repository).. Primary antibodies were as follows: rat anti-mouse Ly-6G antibody 1:100 (Novus Biologicals, Littleton, CO, RB6-8C5), polyclonal rabbit anti-mouse CCL8/MCP-2 antibody 1:100 (antibody-online), and polyclonal rabbit anti-mouse Langerin antibody 1:250 (Novus Biological), controls: rat IgG2b, rabbit IgG.. Primary antibodies were incubated over night at 4 °C (Ly-6G and CCL8) or for 2 hours at room temperature (Langerin).Primary antibodies were incubated over night at 4 °C (Ly-6G and CCL8) or for 2 hours at room temperature (Langerin). |